Longevity Β· NeuralTier 4 β€” Preclinical only

KL1

Klotho fragment Β· Ξ±-Klotho KL1 domain Β· Klotho extracellular domain

The KL1 extracellular domain fragment of the anti-aging protein Ξ±-Klotho. A single peripheral injection of the KL1 fragment restored cognitive function in aged and ADHD mouse models, suggesting a systemic signaling role independent of FGF23 co-receptor activity.

πŸ’‰ SC or IV injection (animal research)🧊 Lyophilised: βˆ’20 Β°C long-term. Reconstituted: βˆ’80 Β°C; single-use aliquots recommended. Avoid freeze-thaw.

Want a personalised protocol?

Our AI generator builds a protocol tailored to your profile and goals.

Generate protocol β†’

Mechanism of Action

Ξ±-Klotho is a transmembrane protein shed as a circulating hormone that declines sharply with age. The KL1 domain appears to cross the blood-brain barrier or signal via peripheral pathways to enhance synaptic plasticity, upregulate GluN2B NMDA receptor subunits in the prefrontal cortex, and reduce oxidative stress. Circulating Klotho levels correlate positively with cognitive performance in humans.

Clinical Applications

  • βœ“Cognitive aging and age-related memory decline (preclinical)
  • βœ“Attention and executive function research (preclinical)
  • βœ“Neuroprotection and synaptic plasticity enhancement (preclinical)
  • βœ“Longevity and healthspan extension research
  • βœ“Potential biomarker role: low Klotho as aging risk indicator

Dosing Protocol

Recommended Dosing

No human dosing established. Primate/mouse studies: single SC or IV injection of 10–250 Β΅g/kg produced cognitive effects lasting days to weeks. Human trials in planning stages. Not for clinical use.

Safety & Contraindications

Possible Side Effects

  • ⚠Unknown safety profile in humans
  • ⚠Theoretical modulation of FGF23/phosphate metabolism with systemic Klotho elevation
  • ⚠Unknown cardiovascular effects at supraphysiological levels

Contraindications

  • βœ•All human use outside approved clinical trials
  • βœ•Chronic kidney disease (Klotho-FGF23 axis dysregulation)
  • βœ•Pregnancy and breastfeeding

Combinations & Synergies

πŸ”— Theoretical synergy with other neuroprotective peptides (Semax, Selank) for cognitive stack
πŸ”— Research interest alongside NAD+ precursors for multi-pathway longevity support